Anti 20S Proteasome Subunit Alpha Type-4 mAb (Clone GC3beta)

Cosmo Bio USA

Anti 20S Proteasome Subunit Alpha Type-4 mAb (Clone GC3beta)

16

Research Use Only

Regulating protein stability and turnover is a key task in the cell. Besides lysosomes, ubiquitin-mediated proteasomal degradation comprises the major proteolytic pathway in eukaryotes. Proteins destined for degradation by the proteasome are conjugated by a tag, a ubiquitin chain to a lysine, through an extensively regulated enzymatic cascade. The ubiquitylated proteins are subsequently targeted for degradation by the 26S proteasome, the major proteolytic machinery for ubiquitylated proteins in the cell. Ubiquitylation can be considered as another covalent post-translational modification and signal, comparable to acetylation, glycosylation, methylation, and phosphorylation. However, ubiquitylation has multiple roles in addition to targeting proteins for degradation. Depending on the number of ubiquitin moieties and the linkages made, ubiquitin also plays an important role in DNA repair, protein sorting and virus budding. Unregulated degradation of proteins, or abnormally stable proteins, interfere with several regulatory pathways, and the ubiquitin-proteasome pathway is affected in a number of diseases, such as neurodegenerative diseases, cellular atrophies and malignancies. Therefore, dissecting the ubiquitin-proteasome pathway and identifying proteins involved in conjunction with the signals required for specific degradation of certain substrates, would help in developing novel therapeutic approaches to treat diseases where the ubiquitin-proteasome pathway is impaired. [from: Roos-Mattjus P. and Sistonen L. The ubiquitin-proteasome pathway (2009) Annals of Medicine 36(4): 285-295] The 26S proteasome is an essential component of the ubiquitin-proteolytic pathway in eukaryotic cells and is responsible for the degradation of most cellular proteins. It is composed of a 20S proteasome catalytic core and regulatory particles at either end. The subunits of the 20S proteasome are classified into two families, alpha and beta. In eukaryotes, the 20S proteasome contains seven alpha-type subunits and seven beta-type subunits. The fourteen subunits are arranged in four rings of seven and form an alpha7beta7beta7alpha7 structure. This antibody recognizes the alpha4 subunit of the 20S proteasome from all organisms tested from yeast to human. References: 1) Tokumoto, M., Horiguchi, R., Nagahama, Y., Tokumoto, T. 1999. Identification of the Xenopus 20S proteasome alpha4 subunit which is modified in the meiotic cell cycle. Gene 239, 301-308. PubMed: 10548731 2) Tokumoto, M., Horiguchi, R., Nagahama, Y., Ishikawa, K., Tokumoto, T. 2000. Two proteins, a goldfish 20S proteasome subunit and the protein interacting with 26S proteasome, change in the meiotic cell cycle. Eur J Biochem 267, 97-103. PubMed: 10601855

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