Anti-3-pHis [SC44-1]

Absolute Antibody

Anti-3-pHis [SC44-1]

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This antibody is specific for 3-pHis and does not cross react with phosphotyrosine (pTyr) or the other pHis isomers.

This antibody can be used for the identification of 3-pHis substrates and functional study of pHis. It was shown to detect various proteins containing 3-pHis, such as SCS and ACLY (Fuhs et al, 2015; pmid: 26140597). SC44-1 was also used for phosphoenrichment using immunoaffinity purification (Hunter et al., 2019). This clone was used to analyse the prevalence of 3-pHis in hepatocellular carcinoma by Western blot (Hindupur et al., 2018; pmid: 29562234). In the same study, SC44-1 was further utilized to demonstrate that LHPP is a protein histidine phosphatase (Hindupur et al., 2018; pmid: 29562234).

Western Blot, Other Application

WB; IAP

Research Use Only

Monoclonal

SC44-1

Rabbit

IgG

Other Species

Species independent

The original rabbit version of this antibody was raised by immunizing rabbits with a 3-pTza library conjugated to the carrier protein keyhole limpet hemocyanin (KLH).

-20°C,2°C to 8°C

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