Anti-4-1BB [4B4-1-1]

Absolute Antibody

Anti-4-1BB [4B4-1-1]

9

This antibody binds to the human 4-1BB, which is expressed on the surface of activated T cells.

The antibody has good binding affinity for Con A-activated T cells and has an immunosuppressive activity towards lymphocyte proliferation in the in vitro mixed lymphocyte reaction. The binding of antibody to surface expressed 4-1BB molecule was assessed using flow cytometry and ELISA was used to check whether the cell culture had binding affinity to 4-1BB (Kim et al., 1995). The interaction of this antibody with CD137 is reported to induce an increase in TNF-alpha production by monocytes. This antibody competes with mAb M121 for binding to CD137 (PMID: 10607752). This antiboy also induces the production of IFN-gamma in NK cells in presence of IL-15 (PMID: 20008791).This antibody can be used in blocking assays and staining methods (PMID: 14746806). Humanized versions of this antibody (Hz4B4-1) and (Hz4B4-2) showed a 1.5 fold and 7.3 fold increase and in binding affinity for 4-1BB as compared to their parental mouse antibody. Additionally, H4B4 mediated antibody-dependent cellular toxicity of activated human peripheral blood T cells and CEM cells in a dose dependent manner (PMID: 11186149). Humanized version of this antibody is available on request.

Flow Cytometry, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking

Research Use Only

Monoclonal

4B4-1-1

TNFRSF9

TNF receptor superfamily member 9

4-1BB; 4-1BB ligand receptor; CD137; CD137 antigen; CDw137; homolog of mouse 4-1BB; ILA; induced by lymphocyte activation (ILA); interleukin-activated receptor, homolog of mouse Ly63; receptor protein 4-1BB; T cell antigen ILA; T-cell antigen 4-1BB homolog; tumor necrosis factor receptor superfamily member 9

Rabbit

IgG

Tumor necrosis factor receptor superfamily member 9

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203