Anti-4Ig-B7H3 [8H9]
AB03824-23.0
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Other Application
Product group Antibodies
ReactivityHuman
TargetCD276
Overview
- SupplierAbsolute Antibody
- Product NameAnti-4Ig-B7H3 [8H9]
- Delivery Days Customer9
- Antibody SpecificityThe antibody recognizes the 4Ig Domain Isoform of the Human B7-Homolog 3, 4Ig-B7H3. The epitope that the antibody recognizes appears to be restricted to tumors versus normal tissues.
- Application Supplier NoteThe antibody was highly reactive with human brain tumors, childhood sarcomas, and neuroblastomas by immunohistochemistry. The antibody was nonreactive with normal human tissues. 4Ig-B7H3 was immunoprecipitated using this antibody (Modak et al., 2001; PMID: 11358824). In vitro characterization of radiolabeled 8H9 showed that (125)I-8H9 had a K(d) of 10.3nM with an estimated 115,000 binding sites on every HTB82 cell. Further, (125)I-8H9 was retained on the cell surface without significant internalization. In vivo targeting of 125I and 131Ilabeled 8H9 in human RMS xenografts was studied. 125I-8H9 could be used for tumor localization in animals. Instead, mice injected with 131I-8H9 showed a significant suppression in tumor volume (Modak et al., 2005; PMID: 16248769). Further, 131-Iodine-8H9 administered through the Ommaya had favorable pharmacokinetics in non-human primates with minimal toxicities. 31-Iodine-8H9 was used in a phase I study with brain tumors, the antibody was safe and might have clinical utility when added to salvage therapy using conventional modalities in the treatment of 8H9-positive LM/CNS cancers. The antibody detected 4Ig-B7H3 under native conditions in LAN-1, HTB82 and U2OS but not in Daudi cells by western blot analysis. The antibody was unable to recognise the antigen under reducing conditions in Western blot analysis (US20100143245A1).
- ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID8H9
- Gene ID80381
- Target nameCD276
- Target descriptionCD276 molecule
- Target synonyms4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD276 antigen; costimulatory molecule
- HostRabbit
- IsotypeIgG
- Protein IDQ5ZPR3
- Protein NameCD276 antigen
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203