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Anti-4Ig-B7H3 [8H9]

AB03824-23.0
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Other Application
Product group Antibodies
ReactivityHuman
TargetCD276
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-4Ig-B7H3 [8H9]
  • Delivery Days Customer
    9
  • Antibody Specificity
    The antibody recognizes the 4Ig Domain Isoform of the Human B7-Homolog 3, 4Ig-B7H3. The epitope that the antibody recognizes appears to be restricted to tumors versus normal tissues.
  • Application Supplier Note
    The antibody was highly reactive with human brain tumors, childhood sarcomas, and neuroblastomas by immunohistochemistry. The antibody was nonreactive with normal human tissues. 4Ig-B7H3 was immunoprecipitated using this antibody (Modak et al., 2001; PMID: 11358824). In vitro characterization of radiolabeled 8H9 showed that (125)I-8H9 had a K(d) of 10.3nM with an estimated 115,000 binding sites on every HTB82 cell. Further, (125)I-8H9 was retained on the cell surface without significant internalization. In vivo targeting of 125I and 131Ilabeled 8H9 in human RMS xenografts was studied. 125I-8H9 could be used for tumor localization in animals. Instead, mice injected with 131I-8H9 showed a significant suppression in tumor volume (Modak et al., 2005; PMID: 16248769). Further, 131-Iodine-8H9 administered through the Ommaya had favorable pharmacokinetics in non-human primates with minimal toxicities. 31-Iodine-8H9 was used in a phase I study with brain tumors, the antibody was safe and might have clinical utility when added to salvage therapy using conventional modalities in the treatment of 8H9-positive LM/CNS cancers. The antibody detected 4Ig-B7H3 under native conditions in LAN-1, HTB82 and U2OS but not in Daudi cells by western blot analysis. The antibody was unable to recognise the antigen under reducing conditions in Western blot analysis (US20100143245A1).
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8H9
  • Gene ID80381
  • Target name
    CD276
  • Target description
    CD276 molecule
  • Target synonyms
    4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD276 antigen; costimulatory molecule
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5ZPR3
  • Protein Name
    CD276 antigen
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203