TOP: Untransfected control; BOTTOM: ARG62337 anti-6X His tag antibody [HIS.H8] (red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (blue).
![Western blot: (1) untransfected control, (2) HEK293 cells transfected with His-tagged protein vector stained with ARG62337 anti-6X His tag antibody [HIS.H8].](https://www.arigobio.com/files/product/5032/ARG62337-WB-1.jpg "Western blot: (1) untransfected control, (2) HEK293 cells transfected with His-tagged protein vector stained with ARG62337 anti-6X His tag antibody [HIS.H8].")
![Western blot: A standard ladder containing five different His-tagged proteins stained with ARG62337 anti-6X His tag antibody [HIS.H8] at 1:2000 (0.5 microg/ml) to compare between.](https://www.arigobio.com/files/product/5032/ARG62337-WB-2.jpg "Western blot: A standard ladder containing five different His-tagged proteins stained with ARG62337 anti-6X His tag antibody [HIS.H8] at 1:2000 (0.5 microg/ml) to compare between.")
TOP: Untransfected control; BOTTOM: ARG62337 anti-6X His tag antibody [HIS.H8] (red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (blue).
anti-6X His tag antibody [HIS.H8]
ARG62337
ApplicationsGel Shift Assay, Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
Overview
- SupplierArigo Biolaboratories
- Product Nameanti-6X His tag antibody [HIS.H8]
- Delivery Days Customer23
- ApplicationsGel Shift Assay, Dot Blot, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHIS.H8
- Concentration1 mg/ml
- ConjugateUnconjugated
- HostMouse
- IsotypeIgG2b
- Scientific DescriptionThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. His-tagged protein is also useful for the scientitists for the binding assay and tracing the location of the protein-interested in the cells when the specific antibodies for the protein-of-interest are unavailable.
- Storage Instruction-20°C
- UNSPSC12352203