Anti-A2a [Fab2838], Human IgG1, kappa

Absolute Antibody

Anti-A2a [Fab2838], Human IgG1, kappa

9

This antibody is specific for the intracellular surface of A(2A)AR.

The original antibody was used for an ELISA, where it showed it was able to bind its antigen. The antibody was used for a ligand binding assay. Here it showed that the antibody was able to stop the binding of agonist NECA but not that of antagonist ZM241385. This suggests that this antibody induces a inactive confirmation of A2AAR. The structure of the original antibody was determined using x-ray crystallography (Hino et al., 2012; PMID:22286059). The heavy chain of this antibody was cloned into a plasmid and HEK293T were transfected. In vitro is showed it was able to block the A2AR signalling pathway (Zhang et al., 2019; PMID:32308059). The protein-protein interaction of this antibody with its antigen was assessed using mCBB CN-PAGE (Suzuki et al., 2018; PMID:29432751).

ELISA, Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

Fab2838

ADORA2A

adenosine A2a receptor

A2aR; adenosine receptor A2a; adenosine receptor subtype A2a; ADORA2; RDC8

Human

IgG1

Adenosine receptor A2a

Human

-20°C,2°C to 8°C

12352203