Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
Figure 1. Western blot analysis of ABCA4 using anti-ABCA4 antibody (PA2066). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse eye tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCA4 antigen affinity purified polyclonal antibody (Catalog # PA2066) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCA4 at approximately 280 kDa. The expected band size for ABCA4 is at 256 kDa.
Anti-ABCA4 Antibody Picoband(r)
PA2066-CY3
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetAbca4
Overview
- SupplierBoster Bio
- Product NameAnti-ABCA4 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID11304
- Target nameAbca4
- Target descriptionATP-binding cassette, sub-family A (ABC1), member 4
- Target synonymsAbc1; Abc10; Abcr; ATP-binding cassette 10; ATP-binding cassette sub-family A member 4; AW050280; D430003I15Rik; R; retinal-specific ATP-binding cassette transporter; retinal-specific phospholipid-transporting ATPase ABCA4; RIM ABC transporter; Rim protein; RmP
- HostRabbit
- IsotypeIgG
- Protein IDO35600
- Protein NameRetinal-specific ATP-binding cassette transporter
- Scientific DescriptionBoster Bio Anti-ABCA4 Antibody catalog # PA2066. Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203