Figure 1. Western blot analysis of ABCB10 using anti-ABCB10 antibody (PB9971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: COLO320 whole cell lysates, Lane 3: 22RV1 whole cell lysates, Lane 4: PANC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB10 antigen affinity purified polyclonal antibody (Catalog # PB9971) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB10 at approximately 79 kDa, 65 kDa. The expected band size for ABCB10 is at 79 kDa.
Figure 1. Western blot analysis of ABCB10 using anti-ABCB10 antibody (PB9971). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat cardiac muscle tissue lysates, Lane 2: COLO320 whole cell lysates, Lane 3: 22RV1 whole cell lysates, Lane 4: PANC whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB10 antigen affinity purified polyclonal antibody (Catalog # PB9971) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCB10 at approximately 79 kDa, 65 kDa. The expected band size for ABCB10 is at 79 kDa.
Anti-ABCB10 Antibody Picoband(r)
PB9971-HRP
ApplicationsWestern Blot
Product group Antibodies
ReactivityChicken, Human, Rat
TargetABCB10
Overview
- SupplierBoster Bio
- Product NameAnti-ABCB10 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateHRP
- Gene ID23456
- Target nameABCB10
- Target descriptionATP binding cassette subfamily B member 10
- Target synonymsABC transporter 10 protein; ABC-me protein; ABC-mitochondrial erythroid protein; ATP-binding cassette sub-family B member 10, mitochondrial; ATP-binding cassette transporter 10; ATP-binding cassette, sub-family B (MDR/TAP), member 10; EST20237; M-ABC2; mitochondrial ATP-binding cassette 2; MTABC2
- HostRabbit
- IsotypeIgG
- Protein IDQ9NRK6
- Protein NameATP-binding cassette sub-family B member 10, mitochondrial
- Scientific DescriptionBoster Bio Anti-ABCB10 Antibody Picoband® catalog # PB9971. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityChicken, Human, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203