Anti-ABCG2 [5D3]

Absolute Antibody

Anti-ABCG2 [5D3]

9

This antibody recognizes and binds a conformation-sensitive external epitope of ABCG2 on living cells. ABCG2 is a broad substrate specificity ATP-dependent transporter from the ATP-binding cassette (ABC) family that actively extrudes a wide variety of phy

This antibody is recommended for identification of human ACBG2 in a sample. This antibody was used in the immunostaining of bone marrow cells to establish a direct link between the SP phenotype and ABCG2 expression (PMID: 11533706). The IgG2b version of this antibody was used to identify AML cell lines that express ABCG2 protein using flow cytometry. This antibody inhibits ABCG2 function, presumably by binding to an external epitope and preventing conformational changes in the transporter, and therefore provides a specific inhibitor assay for ABCG2 function (PMID: 12393637). The SP (side population phenotype) analysis of limbal epithelial cells using 5D3 and TPS-A showed that both 5D3 and TPS-A effectively inhibit Hoechst 33342 dye efflux from these cells (PMID: 15135043). Conformational changes in the ABCG2 multidrug transporter result in the modification of its interaction with 5D3 antibody. This antibody therefore cannot be used to identify human ABCG2 in an immunoblot/western blot assay. The binding of this antibody was maximum in the presence of ATP, ADP, or Ko143, whereas the non-hydrolysable ATP analog, adenosine 5-(beta, gamma-imido)triphosphate (AMP-PNP), and nucleotide trapping by vanadate decreased antibody binding. In membranes expressing the ABCG2-K86M mutant, ATP, ADP, and AMP-PNP decreased, whereas Ko143 increased the binding of this antibody. 5D3 can be used as a sensitive tool to reveal intramolecular changes, reflecting ATP binding, the formation of a catalytic intermediate, or substrate inhibition within the transport cycle of the ABCG2 protein (PMID: 15557326). This antibody was also used in a study involving localization of ABCG2 mitoxantrone resistance-associated protein in normal tissues using immunohistochemistry (PMID: 15990223). This antibody was also used in determining ABCG2 expression using immunofluorescent staining (PMID: 15625123).

Flow Cytometry, ImmunoFluorescence, ImmunoHistoChemistry

Research Use Only

Monoclonal

5D3

ABCG2

ATP binding cassette subfamily G member 2 (Junior blood group)

ABC transporter; ABC15; ABCP; ATP-binding cassette transporter G2; ATP-binding cassette, sub-family G (WHITE), member 2 (Junior blood group); BCRP; BCRP1; BMDP; breast cancer resistance protein; broad substrate specificity ATP-binding cassette transporter ABCG2; CD338; CDw338; EST157481; GOUT1; mitoxantrone resistance-associated protein; MRX; multi drug resistance efflux transport ATP-binding cassette sub-family G (WHITE) member 2; MXR; MXR1; MXR-1; placenta specific MDR protein; placenta-specific ATP-binding cassette transporter; UAQTL1; urate exporter

Mouse

IgG2b

ATP-binding cassette sub-family G member 2

Human

-20°C,2°C to 8°C

12352203