Western blot analysis of ABCG5 using anti-ABCG5 antibody (PA1871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HT1080 Whole Cell Lysate Lane 4: U87 Whole Cell Lysate Lane 5: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (Catalog # PA1871) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73KD. The expected band size for ABCG5 is at 73KD.
Western blot analysis of ABCG5 using anti-ABCG5 antibody (PA1871). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: MCF-7 Whole Cell Lysate Lane 2: A549 Whole Cell Lysate Lane 3: HT1080 Whole Cell Lysate Lane 4: U87 Whole Cell Lysate Lane 5: SKOV Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCG5 antigen affinity purified polyclonal antibody (Catalog # PA1871) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCG5 at approximately 73KD. The expected band size for ABCG5 is at 73KD.
Anti-ABCG5 Antibody Picoband(r)
PA1871
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetABCG5
Overview
- SupplierBoster Bio
- Product NameAnti-ABCG5 Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for ABCG5 is approximately 2.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierWB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID64240
- Target nameABCG5
- Target descriptionATP binding cassette subfamily G member 5
- Target synonymsATP-binding cassette sub-family G member 5; ATP-binding cassette, sub-family G (WHITE), member 5; sterolin 1; STSL; STSL2
- HostRabbit
- IsotypeIgG
- Protein IDQ9H222
- Protein NameATP-binding cassette sub-family G member 5
- Scientific DescriptionBoster Bio Anti-ABCG5 Antibody catalog # PA1871. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Reactivity SupplierHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203