Anti-Abeta (1-40/42) - 4D8 - oligomer specific monoclonal targeting amyloid-beta

Applied Biological Materials

Anti-Abeta (1-40/42) - 4D8 - oligomer specific monoclonal targeting amyloid-beta

9

No confirmed exceptions from predicted reactivity known in the moment

ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

0.5 ug/ul

Mouse

IgM

Alzheimers disease is a progressive neurodegenerative disorder characterized by neurofibrillary tangles and depositionof plaques containing the amyloid-Y (AY) peptide. AY is derived through proteolytic cleavage of the amyloid precursor protein resulting in various peptide lengths corresponding to 38-43 amino acids. AY1-42 is particularly prone to self-aggregate and its aggregates are strongly linked to development of the disease. During in vitro AY self-assembly, highly cytotoxic metastable intermediates (oligomers) are generated ranging from 8 kDa to over 100 kDa. Several reports suggest a similar situation in vivo. We have generated a monoclonal antibody, specific for AY oligomers, by immunizing mice with partly aggregated AY1-40 peptide. The antibody 4D8 is a conformational specific antibody that specifically can detect oligomeric structures of AY (1-42). The reactivity is strongly enhanced within an oligomeric range of approximately 4-15 AY monomers while it shows only very low affinity towards mature fibrils and soluble monomers.

Human

-20°C,2°C to 8°C

12352203