Anti-Acrolein-modified protein [5F6]

Absolute Antibody

Anti-Acrolein-modified protein [5F6]

9

This antibody is specific for acrolein-modified proteins. Protein modification by acrolein is largely attributed to the formation of acrolein-lysine and acrolein-histidine adducts that possess a carbonyl function. While the antibody has been raised agains

A direct ELISA examined the immunoreactivity of this antibody to aldehyde-treated proteins. It was scarcely inhibited by Na-acetyl-Nim-propanal-histidine and significantly inhibited by Na-acetyl-FDP-lysine, indicating that FDP-lysine (Nepsilon-(3-formyl-3,4-dehydropiperidino)lysine) is an epitope. This antibody was also used for immunohistochemistry (IHC) detection of protein-bound acrolein in fatty streak lesions of arterial tissue (Uchida et al., 1998; PMID: 9560197). This antibody was used in flow cytometry (FC) to detect protein-bound acrolein in activated human T cells (Fonseca et al., 2001; PMID: 11342443). A novel pyridinium-type lysine adduct, Nepsilon-(3-methyl-pyridinium)lysine (MP-lysine), was investigated and found to be a major epitope of this antibody (Furuhata et al., 2003; PMID: 14504272). This antibody was used for IHC to detect oxidative stress in the testis of rats (Iuchi et al., 2004; PMID: 14673659). This antibody was used for WB to detect acrolein modification of apoE3-NT (Tamamizu-Kato et al., 2007; PMID: 17580963).

Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

5F6

Rabbit

IgG

Other Species

-20°C,2°C to 8°C

12352203