Belgium

Luxembourg

Netherlands

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Mouse Intestine Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Human Placenta Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Rat Spleen Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Mouse Spleen Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Rat Intestine Tissue

Western blot analysis of Actin using anti-Actin antibody (MA1000). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysate, Lane 2: rat PC-12 whole cell lysate, Lane 3: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # MA1000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 43KD. The expected band size for Actin is at 43KD.

Anti-Actin Antibody (Monoclonal, AC-40) from Boster Bio is available at Bio-Connect

Anti-Actin Antibody (Monoclonal, AC-40) | MA1000 | Boster Bio

Anti-Actin Antibody (Monoclonal, AC-40)

Antibodies

Website

Tech support

Our company

Our team

Divisions

Vacatures

Events

Promotions

About us

Contact

Our people

Chemicals

Proteins / Signaling Molecules

Assays

(Cell) Culture

Products

Our Company

Suppliers

News

Bio-Connect

Biotech, Pharma & CRO

Academia

Diagnostics

Environment

Food

Veterinary

Industries

Disclaimer

Privacy Statement

Terms and conditions

Are you looking for specific products, alternatives, documentation or need assistance with solving product related issues? Let our in-house technical support team help you.

We support your research

<p>For 15 years, we connect life science researchers specialized in the import and distribution of life science reagents from global suppliers.</p>

Support your research

DNA / RNA / Vectors

Cell Culture

Genome Editing and Engineering

Molecular Biology

Lysates / Extracts

Expression

Labware / Accesories

Body Products

Technique Specific Products

Arrays / Slides

Call +31 (0)26 326 44 50

Anti-Actin Antibody (Monoclonal, AC-40)

Large volume orders?

Overview