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Anti-Actin antibody, MA1000, IHC(F) IHC(F): Mouse Intestine Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Human Placenta Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Rat Spleen Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Mouse Spleen Tissue

Anti-Actin antibody, MA1000, IHC(F) IHC(F): Rat Intestine Tissue

Western blot analysis of Actin using anti-Actin antibody (MA1000). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysate, Lane 2: rat PC-12 whole cell lysate, Lane 3: mouse NIH3T3 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Actin antigen affinity purified monoclonal antibody (Catalog # MA1000) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Actin at approximately 43KD. The expected band size for Actin is at 43KD.

Anti-Actin Antibody (Monoclonal, AC-40) from Boster Bio is available at Bio-Connect

Anti-Actin Antibody (Monoclonal, AC-40) | MA1000 | Boster Bio

Anti-Actin Antibody (Monoclonal, AC-40)

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Anti-Actin Antibody (Monoclonal, AC-40)

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