Anti-Adeno associated virus [A20]

Absolute Antibody

Anti-Adeno associated virus [A20]

7

The clone was used to investigate the AAV assembly process on the cellular level. The clone was used to immunoprecipitate capsid proteins from extracts of [35S]methionine-labeled HeLa cells infected with AAV-2 and adenovirus type 2. Immunofluorescence was preformed on AAV-2/adenovirus type 2-coinfected HeLa cells using this antibody (Wistuba et al, 1997; pmid:8995658). Epitope sequences on the capsid surface were identified by enzyme-linked immunoabsorbent assay using AAV-2 mutants and AAV serotypes, peptide scan, and peptide competition experiments. The clone neutralizes infection following receptor attachment by binding an epitope formed during AAV-2 capsid assembly. The antibody bound empty and full AAV-2 particles in an ELISA. In a neutralization assay, incubation of rAAV-2-GFP with A20, on HeLa cells prevented transgene expression. The clone reacted also with AAV-3 as shown by ELISA (Wobus et al, 2000; pmid:10982375). The structure for the complex AAV-2 with Fab fragment was determined by cryo-electron microscopy (McCraw et al, 2012; pmid:22682774).

ImmunoFluorescence, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

IP; IF; ELISA; Neutralizing

Research Use Only

Capsid protein VP1

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203