Anti-Adenylate Cyclase Toxin [9D4]

Absolute Antibody

Anti-Adenylate Cyclase Toxin [9D4]

9

This antibody was generated against Bordetella pertussis adenylate cyclase toxin and recognizes an epitope in the glycine and aspartate-rich nonapeptide repeat region (amino acids 1156-1489) of B. pertussis and other repeats-in-toxin (RTX) family bacteria

In the original study, this antibody was used in conjuction with other 11 mAbs to perform epitope mapping against Bordetella pertussis adenylate cyclase toxin, and to evaluate the roles of these epitopes on toxin function (Lee et al., 1999). This antibody has also been used in various application, such as ELISA, FACS, Western Blotting and inhibition/neutralising assays. Example of functional studies in which this antibody could be utilised include those conducted by Weingart et al. (Infect Immun. 2000) to demonstrate that adenylate cyclase toxin inhibits both Fc receptor-mediated attachment and phagocytosis of B. pertussis by neutrophils, by Martin et al. (Sci Rep. 2015) to delineate the mechanism by which adenylate cyclase toxin promotes bacterial internalisation into non phagocytic cells, and by Finley et al. (PLoS Biol. 2018) to review how an adenylate cyclase toxin uses bait and switch tactics in its activation.

Flow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

9D4

Mouse

IgG2a

Adenylate cyclase toxin

Bacteria

-20°C,2°C to 8°C

12352203