Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Adiponectin using anti-Adiponectin antibody (PB9011). Adiponectin was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Adiponectin Antibody (PB9011) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Adiponectin/ADIPOQ Antibody Picoband(r)
PB9011
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetAdipoq
Overview
- SupplierBoster Bio
- Product NameAnti-Adiponectin Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID246253
- Target nameAdipoq
- Target descriptionadiponectin, C1Q and collagen domain containing
- Target synonymsAcdc; Acrp30; Adid; adipocyte complement related protein of 30 kDa; adiponectin; adiponectin d
- HostRabbit
- IsotypeIgG
- Protein IDQ8K3R4
- Protein Name30 kDa adipocyte complement-related protein
- Scientific DescriptionBoster Bio Anti-Adiponectin/ADIPOQ Antibody Picoband® catalog # PB9011. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityMouse, Rat
- Reactivity SupplierMouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203