Figure 1. Western blot analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADIPOR1 antigen affinity purified polyclonal antibody (Catalog # PB9418) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADIPOR1 at approximately 43 kDa. The expected band size for ADIPOR1 is at 43 kDa.
Figure 1. Western blot analysis of ADIPOR1 using anti-ADIPOR1 antibody (PB9418). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: MCF-7 Whole Cell Lysate at 40ug, Lane 4: A549 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADIPOR1 antigen affinity purified polyclonal antibody (Catalog # PB9418) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADIPOR1 at approximately 43 kDa. The expected band size for ADIPOR1 is at 43 kDa.
Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband(r)
PB9418-PE
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHamster, Human, Rat
TargetADIPOR1
Overview
- SupplierBoster Bio
- Product NameAnti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for ADIPOR1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateRPE
- Gene ID51094
- Target nameADIPOR1
- Target descriptionadiponectin receptor 1
- Target synonymsACDCR1; adiponectin receptor protein 1; CGI45; CGI-45; PAQR1; progestin and adipoQ receptor family member 1; progestin and adipoQ receptor family member I; TESBP1A
- HostRabbit
- IsotypeIgG
- Protein IDQ96A54
- Protein NameAdiponectin receptor protein 1
- Scientific DescriptionBoster Bio Anti-Adiponectin Receptor 1/ADIPOR1 Antibody Picoband® catalog # PB9418. Tested in IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203