Figure 1. Western blot analysis of ADRA1A using anti-ADRA1A antibody (PB9752). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Rat Liver Tissue Lysate at 50ug, Lane 4: Mouse Liver Tissue Lysate at 50ug, Lane 5: Mouse Lung Tissue Lysate at 50ug, Lane 6: 22RV1 Whole Cell Lysate at 40ug, Lane 7: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified polyclonal antibody (Catalog # PB9752) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa.
Figure 1. Western blot analysis of ADRA1A using anti-ADRA1A antibody (PB9752). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Brain Tissue Lysate at 50ug, Lane 3: Rat Liver Tissue Lysate at 50ug, Lane 4: Mouse Liver Tissue Lysate at 50ug, Lane 5: Mouse Lung Tissue Lysate at 50ug, Lane 6: 22RV1 Whole Cell Lysate at 40ug, Lane 7: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADRA1A antigen affinity purified polyclonal antibody (Catalog # PB9752) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ADRA1A at approximately 51 kDa. The expected band size for ADRA1A is at 51 kDa.
Anti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband(r)
PB9752
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetADRA1A
Overview
- SupplierBoster Bio
- Product NameAnti-ADRA1A Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID148
- Target nameADRA1A
- Target descriptionadrenoceptor alpha 1A
- Target synonymsADRA1C; ADRA1L1; alpha-1A adrenergic receptor; alpha-1A adrenoceptor; alpha-1A adrenoreceptor; ALPHA1AAR; alpha-1C adrenergic receptor; G protein coupled receptor
- HostRabbit
- IsotypeIgG
- Protein IDP35348
- Protein NameAlpha-1A adrenergic receptor
- Scientific DescriptionBoster Bio Anti-alpha 1d Adrenergic Receptor/ADRA1A Antibody Picoband® catalog # PB9752. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203