Figure 1. Western blot analysis of AGTR1 using anti-AGTR1 antibody (PB9470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat RH35 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse liver tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (Catalog # PB9470) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
Figure 1. Western blot analysis of AGTR1 using anti-AGTR1 antibody (PB9470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat RH35 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse liver tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (Catalog # PB9470) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband(r)
PB9470
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Monkey, Mouse, Rat
TargetAGTR1
Overview
- SupplierBoster Bio
- Product NameAnti-AGTR1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID185
- Target nameAGTR1
- Target descriptionangiotensin II receptor type 1
- Target synonymsAG2S; AGTR1B; AT1; AT1AR; AT1B; AT1BR; AT1R; AT2R1; HAT1R; type-1 angiotensin II receptor; type-1B angiotensin II receptor
- HostRabbit
- IsotypeIgG
- Protein IDP30556
- Protein NameType-1 angiotensin II receptor
- Scientific DescriptionBoster Bio Anti-Angiotensin II Type 1 Receptor/AGTR1 Antibody Picoband® catalog # PB9470. Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203