IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). AKR1B1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of AKR1B1 using anti-AKR1B1 antibody (PB10035). AKR1B1 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-AKR1B1 Antibody (PB10035) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-AKR1B1 Antibody Picoband(r)
PB10035
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAKR1B1
Overview
- SupplierBoster Bio
- Product NameAnti-AKR1B1 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID231
- Target nameAKR1B1
- Target descriptionaldo-keto reductase family 1 member B
- Target synonymsADR; aldehyde reductase 1; aldo-keto reductase family 1 member B1; aldose reductase; ALDR1; ALR2; AR; Lii5-2 CTCL tumor antigen; low Km aldose reductase
- HostRabbit
- IsotypeIgG
- Protein IDP15121
- Protein NameAldose reductase
- Scientific DescriptionBoster Bio Anti-AKR1B1 Antibody Picoband® catalog # PB10035. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203