Figure 1. Western blot analysis of Aquaporin 1 using anti-Aquaporin 1 antibody (PB9472). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1 antigen affinity purified polyclonal antibody (Catalog # PB9472) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 56 kDa. The expected band size for Aquaporin 1 is at 56 kDa.
Figure 1. Western blot analysis of Aquaporin 1 using anti-Aquaporin 1 antibody (PB9472). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Kidney Tissue Lysate at 50ug, Lane 2: Mouse Kidney Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aquaporin 1 antigen affinity purified polyclonal antibody (Catalog # PB9472) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aquaporin 1 at approximately 56 kDa. The expected band size for Aquaporin 1 is at 56 kDa.
Anti-ALDH2 Antibody Picoband(r)
PB9472
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetALDH2
Overview
- SupplierBoster Bio
- Product NameAnti-ALDH2 Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID217
- Target nameALDH2
- Target descriptionaldehyde dehydrogenase 2 family member
- Target synonymsacetaldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDM; epididymis secretory sperm binding protein; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2
- HostRabbit
- IsotypeIgG
- Protein IDP05091
- Protein NameAldehyde dehydrogenase, mitochondrial
- Scientific DescriptionBoster Bio Anti-ALDH2 Antibody Picoband® catalog # PB9472. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203