Western blot analysis of Aldolase using anti-Aldolase antibody in A549 cell lysate (M05022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aldolase antigen affinity purified polyclonal antibody (Catalog # M05022) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Western blot analysis of Aldolase using anti-Aldolase antibody in A549 cell lysate (M05022). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aldolase antigen affinity purified polyclonal antibody (Catalog # M05022) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Anti-Aldolase ALDOA Monoclonal Antibody
M05022
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-Aldolase Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- FormulationLiquid
- Protein IDP04075
- Protein NameFructose-bisphosphate aldolase A
- Scientific DescriptionBoster Bio Anti-Aldolase ALDOA Monoclonal Antibody catalog # M05022. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
- Storage Instruction-20°C
- UNSPSC12352203