Anti-alpha-Toxin [3A4D10]

Absolute Antibody

Anti-alpha-Toxin [3A4D10]

9

This antibody binds the alpha toxin of Clostridium perfringens. It specifically recognizes and binds the epitope ARGFAK of the toxin.

Alpha toxin is an exotoxin, a type of bacterial haemolysin that attack blood cell membranes and cause cell rupture. Alpha toxin is an essential virulance factor in gas gangrene and causes myonecrosis in infected tissues. ELISA was used to identify the specific clones binding to alpha-toxin and epitope mapping of the antibody. 3A4D10 can even recognize denatrued toxin in a western blot assay. This antibody is capable of neutralizing the toxin in vitro and in vivo assays (PMID: 1718874). Only immunization with amino acids 247-370 of the Clostridium perfringens alpha toxin induced protection against the lethal effects of the toxin. The toxin plays a major role in pathogenesis of gas gangrene (PMID: 825650). Humanized version derived from its murine parent showed higher binding affinity (PMID: 7716162).

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

3A4D10

Mouse

IgG1

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203