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ProductsBack/Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)
Figure 1. IHC analysis of alpha-Tubulin using anti-alpha-Tubulin antibody (MA1107). alpha-Tubulin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml mouse anti-alpha-Tubulin Antibody (MA1107) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 1. IHC analysis of alpha-Tubulin using anti-alpha-Tubulin antibody (MA1107). alpha-Tubulin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml mouse anti-alpha-Tubulin Antibody (MA1107) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Figure 1. IHC analysis of alpha-Tubulin using anti-alpha-Tubulin antibody (MA1107). alpha-Tubulin was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml mouse anti-alpha-Tubulin Antibody (MA1107) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.

Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A)

MA1107
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetTUBA1A
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Alpha-Tubulin Antibody (Monoclonal, DM1A)
  • Delivery Days Customer
    9
  • Application Supplier Note
    Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHP, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DM1A
  • Concentration
    100 ug/ml
  • Gene ID7846
  • Target name
    TUBA1A
  • Target description
    tubulin alpha 1a
  • Target synonyms
    B-ALPHA-1, LIS3, TUBA3, tubulin alpha-1A chain, hum-a-tub1, hum-a-tub2, tubulin B-alpha-1, tubulin alpha-3 chain, tubulin, alpha, brain-specific
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ71U36
  • Protein Name
    Tubulin alpha-1A chain
  • Scientific Description
    Boster Bio Anti-Alpha-Tubulin TUBA1A Antibody (Monoclonal, DM1A) catalog # MA1107. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.
  • Reactivity Supplier
    Chicken, Human, Mouse, Rat, Chicken
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wang F, Zhang S, Sun F, et al. Anti-angiogenesis and anti-immunosuppression gene therapy through targeting COUP-TFII in an in situ glioblastoma mouse model. Cancer Gene Ther. 2024,31(8):1135-1150. doi: 10.1038/s41417-024-00799-z
    Read this paper
  • Li X, Yuan F, Xiong Y, et al. FAM3A plays a key role in protecting against tubular cell pyroptosis and acute kidney injury. Redox Biol. 2024,74:103225. doi: 10.1016/j.redox.2024.103225
    Read this paper
  • Huang Q, Liu L, Tan X, et al. Empagliflozin alleviates neuroinflammation by inhibiting astrocyte activation in the brain and regulating gut microbiota of high-fat diet mice. J Affect Disord. 2024,360:229-241. doi: 10.1016/j.jad.2024.05.150
    Read this paper
  • Song X, Li D, Gan L, et al. Intravenous Injection of Na Ions Aggravates Ang II-Induced Hypertension-Related Vascular Endothelial Injury by Increasing Transmembrane Osmotic Pressure. Int J Nanomedicine. 2023,18:7505-7521. doi: 10.2147/IJN.S435144
    Read this paper
  • Li H, Zhu L, Weng Z, et al. Sesamin attenuates UVA-induced keratinocyte injury via inhibiting ASK-1-JNK/p38 MAPK pathways. J Cosmet Dermatol. 2024,23(1):316-325. doi: 10.1111/jocd.15951
    Read this paper
  • Song J, Liu S, Ren Y, et al. Organotin benzohydroxamate derivatives (OTBH) target colchicine-binding site exerting potent antitumor activity both in vitro and vivo revealed by quantitative proteomic analysis. Eur J Pharm Sci. 2023,187:106488. doi: 10.1016/j.ejps.2023.106488
    Read this paper
  • Dong H, Zeng L, Chen W, et al. N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness. Cell Death Discov. 2023,9(1):149. doi: 10.1038/s41420-023-01442-w
    Read this paper
  • Hu Y, Xie Q, Wu X, et al. Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration. Cell Death Dis. 2022,13(10):856. doi: 10.1038/s41419-022-05306-6
    Read this paper
  • Zhang J, Hao N, Li W, et al. Simvastatin Upregulates Lipoxin A4 and Accelerates Neuroinflammation Resolution After Intracerebral Hemorrhage. Curr Neurovasc Res. 2022,19(3):321-332. doi: 10.2174/1567202619666220913124627
    Read this paper
  • Huang J, Xu Z, Chen H, et al. Shen Qi Wan Ameliorates Learning and Memory Impairment Induced by STZ in AD Rats through PI3K/AKT Pathway. Brain Sci. 2022,12(6). doi: 10.3390/brainsci12060758
    Read this paper

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Figure 1. Western blot analysis of Tubulin alpha using anti-Tubulin alpha antibody (A03989-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tubulin alpha antigen affinity purified polyclonal antibody (Catalog # A03989-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tubulin alpha at approximately 50 kDa. The expected band size for Tubulin alpha is at 50 kDa.
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