Anti-alpha-V beta 3 integrin [EM22703]

Absolute Antibody

Anti-alpha-V beta 3 integrin [EM22703]

7

This antibody has been used to specifically bind alphavbeta3 in Western blots and in flow cytometry, and to specifically stain alphavbeta3 integrins in FFPE (formalin fixed paraffin embedded)/TMA material, where murine antibodies function poorly (Goodman et al, 2012; Hebeta et al., 2014; Roth et al, 2013). alphavbeta3 was found to be strongly expressed in some invasive NSCLC samples, though was not expressed by PC3 prostate cells and human prostate tumours. In FC, the EM22703 signal is increased 3-10 fold in the presence of nanomolar concentrations of the antagonistic alphavbeta3 inhibitor cilengitide, indicating that EM22703 detects a ligand-induced binding site on alphavbeta3 (Goodman et al, 2012).

Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

-20°C,2°C to 8°C

12352203