Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
Anti-AMH Antibody Picoband(r)
A00763-CY3
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetAMH
Overview
- SupplierBoster Bio
- Product NameAnti-AMH Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateCy3
- Gene ID268
- Target nameAMH
- Target descriptionanti-Mullerian hormone
- Target synonymsanti-Muellerian hormone; MIF; MIS; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian inhibiting factor; Mullerian inhibiting substance
- HostRabbit
- IsotypeIgG
- Protein IDP03971
- Protein NameMuellerian-inhibiting factor
- Scientific DescriptionBoster Bio Anti-AMH Antibody Picoband® catalog # A00763. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203