Figure 2. IHC analysis of Amylase using anti-Amylase antibody (A12967). Amylase was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Amylase Antibody (A12967) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Amylase using anti-Amylase antibody (A12967). Amylase was detected in paraffin-embedded section of human pancreatic cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Amylase Antibody (A12967) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Amylase/AMY1A/AMY1B/AMY1C Antibody Picoband(r)
A12967-IFLUOR647
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAMY1A
Overview
- SupplierBoster Bio
- Product NameAnti-Amylase/AMY1A/AMY1B/AMY1C Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateOther Conjugate
- Gene ID276
- Target nameAMY1A
- Target descriptionamylase alpha 1A
- Target synonyms1,4-alpha-D-glucan glucanohydrolase 1; alpha-amylase 1; alpha-amylase 1A; AMY1; amylase alpha 1A (salivary); amylase, salivary, alpha-1A; glycogenase; salivary alpha-amylase; salivary amylase alpha 1A
- HostRabbit
- IsotypeIgG
- Protein IDP04745
- Protein NameAlpha-amylase 1A
- Scientific DescriptionBoster Bio Anti-Amylase/AMY1A/AMY1B/AMY1C Antibody Picoband® catalog # A12967. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203