Anti-Amyloid beta [scFv59]

Absolute Antibody

Anti-Amyloid beta [scFv59]

9

This antibody is specific for oligomeric as well as fibrillar/aggregated Abeta.

The scFv version of this antibody demonstrated the highest ELISA titer for Abetaimmunoreactivity against Abeta1-42, and its specificity for oligomeric and fibrillar/aggregated Abeta was demonstrated in a WB. The scFv version of this antibody reacted with amyloid beta deposits in brain sections from a Tg2576 mouse and in skeletal muscle sections from a Tg13592 mouse, as observed via IHC. The scFv version of this antibody was used to inhibit the formation of Abeta fibril formation in vitro as demonstrated in a Thioflavin T assay. This inhibition contributed to the amelioration of Abeta cytotoxicity as was shown in vitro in an MTT assay; cell viability increased from 45.2 +/- 0.78% to 58.8 +/- 5.45% (P = 0.069) in the presence of scFv59. Furthermore, Tg2576 mice subjected to scFv59 injection in the hippocampus and cortex had fewer diffuse and fibrillar Abeta deposits in those areas than Tg2576 mice injected with PBS (Fukuchi et al., 2006; PMID: 16630540). Vascular amyloid was also stained by scFv59 (Fukuchi et al., 2006; PMID: 16766200).

Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

scFv59

APP

amyloid beta precursor protein

AAA; ABETA; ABPP; AD1; alpha-sAPP; alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid precursor protein; amyloid-beta precursor protein; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; cerebral vascular amyloid peptide; CTFgamma; CVAP; peptidase nexin-II; PN2; PN-II; preA4; protease nexin-II; testicular tissue protein Li 2

Human

IgG1

Amyloid-beta A4 protein

Human, Mouse

-20°C,2°C to 8°C

12352203