Figure 1. IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody (A00370). Angiopoietin-2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Angiopoietin-2 Antibody (A00370) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1. IHC analysis of Angiopoietin-2 using anti-Angiopoietin-2 antibody (A00370). Angiopoietin-2 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Angiopoietin-2 Antibody (A00370) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Angiopoietin-2/ANGPT2 Antibody Picoband(r)
A00370-10UG
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetANGPT2
Overview
- SupplierBoster Bio
- Product NameAnti-Angiopoietin-2/ANGPT2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID285
- Target nameANGPT2
- Target descriptionangiopoietin 2
- Target synonymsAGPT2; ANG2; angiopoietin-2; angiopoietin-2a; angiopoietin-2B; LMPHM10; Tie2-ligand
- HostRabbit
- IsotypeIgG
- Protein IDO15123
- Protein NameAngiopoietin-2
- Scientific DescriptionBoster Bio Anti-Angiopoietin-2/ANGPT2 Antibody Picoband® catalog # A00370. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203