IHC analysis of APC using anti-APC antibody (M00008) in human colon cancer samples. APC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APC Antibody (M00008) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of APC using anti-APC antibody (M00008) in human colon cancer samples. APC was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-APC Antibody (M00008) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-APC Monoclonal Antibody
M00008
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPC
Overview
- SupplierBoster Bio
- Product NameAnti-APC Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDFAD-1
- FormulationLiquid
- Gene ID324
- Target nameAPC
- Target descriptionAPC regulator of WNT signaling pathway
- Target synonymsadenomatosis polyposis coli tumor suppressor; adenomatous polyposis coli (APC); adenomatous polyposis coli protein; APC, WNT signaling pathway regulator; BTPS2; deleted in polyposis 2.5; DESMD; DP2; DP2.5; DP3; epididymis secretory sperm binding protein; GS; PPP1R46; protein phosphatase 1, regulatory subunit 46; truncated adenomatosis polyposis coli; WNT signaling pathway regulator
- HostRabbit
- IsotypeIgG
- Protein IDP25054
- Protein NameAdenomatous polyposis coli protein
- Scientific DescriptionBoster Bio Anti-APC Monoclonal Antibody catalog # M00008. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203