Anti-Apo E [1D7]

Absolute Antibody

Anti-Apo E [1D7]

7

Is specific for the human apolipoprotein E antigen, and appears to be sensitive to lysine modifications and binds very poorly to carbamylated Apo E. The epitope corresponds to the LDLr binding site on Apo E, and incorporation into lipid vesicles does not

The binding specificity of this antibody has been confirmed using a radioimmunoassay showing it to be specific to Apo E using a radioimmunoassay which is sensitive to carbamylation and methlyation (Milne et al, 1981). Surface plasmon resonance has been used to analyse the kinetics of the binding of 1D7 (Raffai et al, 1995). Sandwich assays have also been used to determine the epitope 1D7 binds to.

ELISA, Other Application, RadioImmunoAssay

RIA; SPR; ELISA

Research Use Only

Monoclonal

1D7

APOE

apolipoprotein E

AD2; APO-E; ApoE4; apolipoprotein E; apolipoprotein E3; LDLCQ5; LPG

Human

IgG1

Apolipoprotein E

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

BALB/c mice were used, were injected twice with 50 ug of purified apo E 1 week apart.

-20°C,2°C to 8°C

12352203