Figure 2. IHC analysis of APP using anti-APP antibody (PB9091). APP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of APP using anti-APP antibody (PB9091). APP was detected in paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-APP Antibody (PB9091) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-beta Amyloid/APP Antibody Picoband(r)
PB9091
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetAPP
Overview
- SupplierBoster Bio
- Product NameAnti-APP Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for APP is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID351
- Target nameAPP
- Target descriptionamyloid beta precursor protein
- Target synonymsAAA; ABETA; ABPP; AD1; alpha-sAPP; alzheimer disease amyloid protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid precursor protein; amyloid-beta precursor protein; APPI; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; cerebral vascular amyloid peptide; CTFgamma; CVAP; peptidase nexin-II; PN2; PN-II; preA4; protease nexin-II; testicular tissue protein Li 2
- HostRabbit
- IsotypeIgG
- Protein IDP05067
- Protein NameAmyloid-beta A4 protein
- Scientific DescriptionBoster Bio Anti-beta Amyloid/APP Antibody Picoband® catalog # PB9091. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Equine, Human, Monkey, Mouse, Rabbit, Rat
- Reactivity SupplierHuman, Mouse, Rat, Bovine, Horse, Monkey, Rabbit
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203