Anti-Aquaporin 4 [C9401]

Absolute Antibody

Anti-Aquaporin 4 [C9401]

7

This antibody recognizes and binds extracellular domains of both the M1 and M23 isoforms of human aquaporin 4 (AQP4), but not mouse aquaporin 4. AQP4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial

This antibody binds extracellular domain of human aquaporin 4 with a very high binding affinity. The binding of this antibody inhibits the binding of NMO-IgG to AQP4 (PMID: 23746426). The binding affinity of this antibody for human AQP4 M23 isoform expressed on the surface of baculovirus and CHO cells was tested with ELISA (PMID: 26398585; WO2014196658). The binding characterization of this antibody was done using flow cytometry. This antibody can be used for the immunoprecipitation of human AQP4 but not mouse AQP4 (WO2014196658; PMID: 23909421). This antibody is not suitable for detection of denatured human AQP4 in a western blot assay (WO2014196658).

Flow Cytometry, ImmunoPrecipitation, ELISA, Neutralisation/Blocking

ELISA; FC; IP; inhibit

Research Use Only

Monoclonal

C9401

AQP4

aquaporin 4

aquaporin type4; aquaporin-4; mercurial-insensitive water channel; MIWC; WCH4

Mouse

IgG2b

Aquaporin-4

Human

Human

The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus.

-20°C,2°C to 8°C

12352203