Figure 1. Western blot analysis of ARHGAP45 using anti-ARHGAP45 antibody (A31863-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGAP45 antigen affinity purified polyclonal antibody (Catalog # A31863-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGAP45 at approximately 160 kDa. The expected band size for ARHGAP45 is at 125 kDa.
Figure 1. Western blot analysis of ARHGAP45 using anti-ARHGAP45 antibody (A31863-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARHGAP45 antigen affinity purified polyclonal antibody (Catalog # A31863-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARHGAP45 at approximately 160 kDa. The expected band size for ARHGAP45 is at 125 kDa.
Anti-ARHGAP45 Antibody Picoband(r)
A31863-1
TargetARHGAP45
Product group Antibodies
Overview
- SupplierBoster Bio
- Product NameAnti-ARHGAP45 Antibody Picoband(r)
- Delivery Days Customer9
- Concentration500 ug/ml
- Gene ID23526
- Target nameARHGAP45
- Target descriptionRho GTPase activating protein 45
- Target synonymsHA-1; histocompatibility (minor) HA-1; HLA-HA1; HMHA1; minor histocompatibility antigen HA-1; minor histocompatibility protein HA-1; rho GTPase-activating protein 45
- Protein IDQ92619
- Protein NameRho GTPase-activating protein 45
- Scientific DescriptionBoster Bio Anti-ARHGAP45 Antibody Picoband® catalog # A31863-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203