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Figure 1. Immunohistochemistry validation of TRIP4 using Anti-ASC-1 (G105) TRIP4 Antibody (A07762). Immunohistochemistry (IHC) analyzes of ASC-1 (G105) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
Figure 1. Immunohistochemistry validation of TRIP4 using Anti-ASC-1 (G105) TRIP4 Antibody (A07762). Immunohistochemistry (IHC) analyzes of ASC-1 (G105) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody
Figure 1. Immunohistochemistry validation of TRIP4 using Anti-ASC-1 (G105) TRIP4 Antibody (A07762). Immunohistochemistry (IHC) analyzes of ASC-1 (G105) pAb in paraffin-embedded human colon carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right)Using PBS instead of primary antibody

Anti-ASC-1 (G105) TRIP4 Antibody

A07762
Boster Bio
ApplicationsImmunoFluorescence, ImmunoHistoChemistry
Product group Antibodies
TargetTRIP4
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-ASC-1 (G105) TRIP4 Antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID9325
  • Target name
    TRIP4
  • Target description
    thyroid hormone receptor interactor 4
  • Target synonyms
    activating signal cointegrator 1; ASC1; ASC-1; HsT17391; MDCDC; SMABF1; thyroid receptor-interacting protein 4; TR-interacting protein 4; TRIP-4; ZC2HC5; zinc finger, C2HC5-type
  • Host
    Rabbit
  • Protein IDQ15650
  • Protein Name
    Activating signal cointegrator 1
  • Scientific Description
    Boster Bio Anti-ASC-1 (G105) TRIP4 Antibody catalog # A07762. Tested in IHC,IF applications. This antibody reacts with Human,Mouse.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASC1/TRIP4 using anti-ASC1/TRIP4 antibody (A07762-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC1/TRIP4 antigen affinity purified polyclonal antibody (Catalog # A07762-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC1/TRIP4 at approximately 68 kDa. The expected band size for ASC1/TRIP4 is at 66 kDa.
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