Figure 1. Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa.
Figure 1. Western blot analysis of B7 DC using anti-B7 DC antibody (PB10010). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-B7 DC antigen affinity purified polyclonal antibody (Catalog # PB10010) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for B7 DC at approximately 37 kDa. The expected band size for B7 DC is at 31 kDa.
Anti-B7 DC/PDCD1LG2 Antibody Picoband(r)
PB10010
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPDCD1LG2
Overview
- SupplierBoster Bio
- Product NameAnti-B7 DC Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID80380
- Target namePDCD1LG2
- Target descriptionprogrammed cell death 1 ligand 2
- Target synonymsB7 dendritic cell molecule; B7DC; B7-DC; bA574F11.2; Btdc; butyrophilin B7-DC; CD273; PD-1-ligand 2; PDCD1 ligand 2; PDCD1L2; PDL2; PD-L2; programmed cell death 1 ligand 2; programmed death ligand 2
- HostRabbit
- IsotypeIgG
- Protein IDQ9BQ51
- Protein NameProgrammed cell death 1 ligand 2
- Scientific DescriptionBoster Bio Anti-B7 DC/PDCD1LG2 Antibody Picoband® catalog # PB10010. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203