Anti-B7-H4 [6H3]

Absolute Antibody

Anti-B7-H4 [6H3]

7

This antibody is specific for the IgC region of human B7-H4.

Humanized variants of this antibody 6H3V6 and 6H3V7 are available on request. This antibody is able to neutralize the effect of an B7-H4 blocking antibody (US9574000). Flow cytometry was performed on HEK293 cells that expressed full length human B7-H4 using this antibody (US9574000). This antibody induces robust internalization of B7-H4 expressed on the surface of both the B7-H4 transfected cell line and the B7-H4 positive breast cancer cell line SK-BR-3 (US9574000). Immunohistochemistry was performed on human tissue using this antibody (US9574000). An internalization assay was performed using this antibody on EG7 (B7-H4 negative cells), EG7.IG7 (B7-H4 low expressing cells), and EG7.IVB3 (B7-H4 high expressing cells) (US9574000). Antibody-Dependent Cell-Mediated Cytotoxicity assay was performed on EG7.hB7-H4 (clone IVB3) cells and SK-BR-3 cells using this antibody (US9574000). An ELISA was performed on B7-H4ECD his-tagged protein using this antibody (US9574000).

Functional Assay, Flow Cytometry, ELISA, ImmunoHistoChemistry

ELISA; FC; IHC; functional assay

Research Use Only

Monoclonal

6H3

VTCN1

V-set domain containing T cell activation inhibitor 1

B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7h.5; B7H4; B7-H4; B7S1; B7X; immune costimulatory protein B7-H4; PRO1291; T cell costimulatory molecule B7x; VCTN1; V-set domain-containing T-cell activation inhibitor 1

Human

IgG1

V-set domain-containing T-cell activation inhibitor 1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

The original antibody was raised by immunizing a DO11.10 mouse with B7-H4.

-20°C,2°C to 8°C

12352203