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Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-Bax Antibody Picoband(r)

A00183
Boster Bio
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetBAX
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Bax Picoband Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    IHP, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID581
  • Target name
    BAX
  • Target description
    BCL2 associated X, apoptosis regulator
  • Target synonyms
    BCL2L4, apoptosis regulator BAX, BCL2 associated X protein, BCL2-associated X protein omega, Baxdelta2(G8)-RFS protein, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, bcl-2-like protein 4, bcl2-L-4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07812
  • Protein Name
    Apoptosis regulator BAX
  • Scientific Description
    Boster Bio Anti-Bax Antibody Picoband® catalog # A00183. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Sun J, Chen H, Xu X, et al. Effect of maternal cigarette smoke exposure on COPD progression in offspring mice. Reprod Toxicol. 2024,128:108646. doi: 10.1016/j.reprotox.2024.108646
    Read this paper
  • Zhu Y, Zhang S, Shao Y, et al. Regulatory role of oxidative stress in retrorsine - Induced apoptosis and autophagy in primary rat hepatocytes. Ecotoxicol Environ Saf. 2024,279:116515. doi: 10.1016/j.ecoenv.2024.116515
    Read this paper
  • Chen F, Yu X. Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability. Cardiovasc Toxicol. 2024,24(7):625-636. doi: 10.1007/s12012-024-09865-2
    Read this paper
  • Zheng H, Liang G, Guan C, et al. Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study. Nanomaterials (Basel). 2024,14(8). doi: 10.3390/nano14080689
    Read this paper
  • Wu J, Cui Y, Ding W, et al. The protective effect of Macrostemonoside T from Allium macrostemon Bunge against Isoproterenol-Induced myocardial injury via the PI3K/Akt/mTOR signaling pathway. Int Immunopharmacol. 2024,133:112086. doi: 10.1016/j.intimp.2024.112086
    Read this paper
  • Li Y, Fan F, Liu Q. Cytisine-N-methylene-(5,7,4(')-trihydroxy)- isoflavone ameliorates ischemic stroke-induced brain injury in mouse by regulating the oxidative stress and BDNF-Trkb/Akt pathway. Eur J Pharmacol. 2024,974:176512. doi: 10.1016/j.ejphar.2024.176512
    Read this paper
  • Xiao M, Pu X, Tong W, et al. Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-β receptor type 2 axis. Cell Mol Biol (Noisy-le-grand). 2024,70(2):143-149. doi: 10.14715/cmb/2024.70.2.20
    Read this paper
  • Wang H, Li C, Zhu L, et al. Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis. In Vitro Cell Dev Biol Anim. 2024,60(7):805-814. doi: 10.1007/s11626-024-00857-8
    Read this paper
  • Zhang Y, Gao X, Cao M, et al. Hepatitis E virus causes apoptosis of ovarian cells in hens and resulting in a decrease in egg production. Poult Sci. 2024,103(4):103501. doi: 10.1016/j.psj.2024.103501
    Read this paper
  • Liu QH, Zhang K, Feng SS, et al. Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways. Int J Mol Sci. 2024,25(3). doi: 10.3390/ijms25031875
    Read this paper

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Figure 5. IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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