IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of Bax using anti-Bax antibody (A00183). Bax was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bax Antibody (A00183) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Bax Antibody Picoband(r)
A00183
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBAX
Overview
- SupplierBoster Bio
- Product NameAnti-Bax Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
- Applications SupplierIHP, WB, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID581
- Target nameBAX
- Target descriptionBCL2 associated X, apoptosis regulator
- Target synonymsapoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
- HostRabbit
- IsotypeIgG
- Protein IDQ07812
- Protein NameApoptosis regulator BAX
- Scientific DescriptionBoster Bio Anti-Bax Antibody Picoband® catalog # A00183. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Reactivity SupplierHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
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- MiR-153 downregulation alleviates PTSD-like behaviors and reduces cell apoptosis by upregulating the Sigma-1 receptor in the hippocampus of rats exposed to single-prolonged stress. Chen YL et al., 2022 Jun, Exp NeurolRead more
- Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders. Zhao T et al., 2022 Feb 15, J Hazard MaterRead more
- Ferritinophagy-Mediated ROS Production Contributed to Proliferation Inhibition, Apoptosis, and Ferroptosis Induction in Action of Mechanism of 2-Pyridylhydrazone Dithiocarbamate Acetate. Li L et al., 2021, Oxid Med Cell LongevRead more
- Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway. Xu Y et al., 2021, Drug Des Devel TherRead more
- Allicin suppresses growth and metastasis of gastric carcinoma: the key role of microRNA-383-5p-mediated inhibition of ERBB4 signaling. Lv Q et al., 2020 Oct, Biosci Biotechnol BiochemRead more
- Clioquinol improves motor and non-motor deficits in MPTP-induced monkey model of Parkinsons disease through AKT/mTOR pathway. Shi L et al., 2020 May 18, Aging (Albany NY)Read more
- Antihypertensive and renal protective effect of Shunaoxin pill combined with captopril on spontaneous hypertension rats. Man S et al., 2020 May, Biomed PharmacotherRead more