Anti-BCL2 [bcl-2/100]

Absolute Antibody

Anti-BCL2 [bcl-2/100]

7

This antibody recognises human BLC-2 which is an antiapoptotic factor in various cell types. Its disregulation was implicated in different conditions, such as cancers and autoimmune disorders.

Bcl-2 has been implicated in various cancers, such as lymphoma, melanoma or colorectal cancer. Therefore, multiple immunohistochemical stainings against bcl-2 protein were performed and this antibody was found to be useful in characterisation of molecular features of certain cancers. ICH: One group used bcl-2/100 antibody to stain paraffin-embedded colorectal cancer samples and strong signal, mainly from the colonic cells, was observed in the tissue (Nicholson et al., 2014). Another group noted bcl-2 immunohistochemical staining in both morular and nonmorular papillary thyroid carcinoma samples while trying to identify cliinicopathological features of this carcinoma (Jung et al., 2009). Finally, yet another found that neoplastic cells of the primary sclerosing epithelioid fibrosarcoma (SEF) of the kidney are characterised by a strong anti-bcl2 immunohistochemical staining with this antibody (Ertoy Baydar et al., 2015). WB: Additionally, multiple groups were successfull while using bcl-2/100 antibody to detect bcl-2 via Western blot; for example in HEC cells while investigating the influence of simvastatin combined with kallistatin on paediatric burn patients with sepsis (Dai and Zhao, 2018).

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

IHC; WB; IP

Research Use Only

Monoclonal

bcl-2/100

BCL2

BCL2 apoptosis regulator

apoptosis regulator Bcl-2; B-cell CLL/lymphoma 2; Bcl-2; PPP1R50; protein phosphatase 1, regulatory subunit 50

Human

IgG1

Apoptosis regulator Bcl-2

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

This antibody was raised against a synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein.

-20°C,2°C to 8°C

12352203