IHC analysis of BDNF using anti-BDNF antibody (PB9075). BDNF was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
IHC analysis of BDNF using anti-BDNF antibody (PB9075). BDNF was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-BDNF Antibody Picoband(r)
PB9075
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Monkey, Mouse, Rabbit, Rat
TargetBdnf
Overview
- SupplierBoster Bio
- Product NameAnti-BDNF Picoband Antibody
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- Application Supplier NoteWB: The detection limit for BDNF is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierFlow Cytometry, IHC, ICC, WB
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- FormulationLyophilized
- Gene ID12064
- Target nameBdnf
- Target descriptionbrain derived neurotrophic factor
- Target synonymsbrain-derived neurotrophic factor
- HostRabbit
- IsotypeIgG
- Protein IDP21237
- Protein NameBrain-derived neurotrophic factor
- Scientific DescriptionBoster Bio Anti-BDNF Antibody Picoband® catalog # PB9075. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityBovine, Canine, Equine, Human, Monkey, Mouse, Rabbit, Rat
- Reactivity SupplierHuman, Mouse, Rat
- Reactivity Supplier NoteHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo. Wang R et al., 2022 Sep 30, Int J Biol MacromolRead more
- SPIONs mediated magnetic actuation promotes nerve regeneration by inducing and maintaining repair-supportive phenotypes in Schwann cells. Liu T et al., 2022 Mar 27, J NanobiotechnologyRead more
- Electroacupuncture Ameliorates Learning and Memory and Improves Synaptic Plasticity via Activation of the PKA/CREB Signaling Pathway in Cerebral Hypoperfusion. Zheng CX et al., 2016, Evid Based Complement Alternat MedRead more
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