Anti-Bet v 1 [H3-1]

Absolute Antibody

Anti-Bet v 1 [H3-1]

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This antibody reacts with native Bet v 1 and homologous allergens. This antibody can also bind Bet v 1-related allergens namely Aln g 1, Cor a 1, Mal d 1. This antibody specifically binds C-terminal F2 domain of Bet v 1 (amino acids 130-160) and the bindi

The reactivity of this antibody to Bet v 1 and related allergens Aln g 1, Cor a 1, Mal d 1 was determined using ELISA. This antibody reacted best with rBet v 1 but also with the cross-reactive allergens from alder (Aln g 1), hazel (Cor a 1), and apple (Mal d 1). The original scFv version of this antibody binds Bet v 1, Aln g 1, Cor a 1, and Mal d 1 with a binding affinity of Bet v 1: KD= 6.22 x 10-10 M, Mal d 1: KD= 2.57 x 10-9 M, Aln g 1: KD= 2.96 x 10-9 M and Cor a 1: KD= 6.83 x 10-9 M respectively (PMID: 29315611). This antibody inhibited allergic patients polyclonal IgE binding to Bet v 1, and partially suppressed allergen-induced basophil activation (PMID: 29315611).

ELISA

ELISA;

Research Use Only

Monoclonal

H3-1

Rabbit

IgG

Major pollen allergen Bet v 1-A

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

Plant

Betula pendula (European white birch), Betula verrucosa, Alnus glutinosa (European alder), Betula alnus var. glutinosa, Corylus avellana (European hazel), Corylus maxima, Malus domestica (Apple), Pyrus malus

The original antibody was isolated from a phage‐displayed combinatorial single‐chain fragment (ScFv) library generated from the peripheral blood mononuclear cells of an immunized subject and screened for Bet v 1‐reactive antibody fragments.

-20°C,2°C to 8°C

12352203