Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using beta III Tubulin Antibody(M01857-1) TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (M01857-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human thyroid cancer, using beta III Tubulin Antibody(M01857-1) TUBB3 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TUBB3 Antibody (M01857-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody
M01857-1
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTUBB3
Overview
- SupplierBoster Bio
- Product NameAnti-beta III Tubulin Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDCC-20
- FormulationLiquid
- Gene ID10381
- Target nameTUBB3
- Target descriptiontubulin beta 3 class III
- Target synonymsbeta-4; CDCBM; CDCBM1; CFEOM3; CFEOM3A; class III beta-tubulin; FEOM3; TUBB4; tubulin beta-3 chain; tubulin beta-4 chain; tubulin beta-III; tubulin, beta 3
- HostRabbit
- IsotypeIgG
- Protein IDQ13509
- Protein NameTubulin beta-3 chain
- Scientific DescriptionBoster Bio Anti-beta III Tubulin TUBB3 Rabbit Monoclonal Antibody catalog # M01857-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC12352203