Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody(M00321) BMP4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BMP4 Antibody (M00321)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody(M00321) BMP4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BMP4 Antibody (M00321)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-BMP4 Rabbit Monoclonal Antibody
M00321
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetBMP4
Overview
- SupplierBoster Bio
- Product NameAnti-BMP4 Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDBBB-2
- FormulationLiquid
- Gene ID652
- Target nameBMP4
- Target descriptionbone morphogenetic protein 4
- Target synonymsBMP2B; BMP2B1; bone morphogenetic protein 2B; bone morphogenetic protein 4; MCOPS6; OFC11; ZYME
- HostRabbit
- IsotypeIgG
- Protein IDP12644
- Protein NameBone morphogenetic protein 4
- Scientific DescriptionBoster Bio Anti-BMP4 Rabbit Monoclonal Antibody catalog # M00321. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203
References
- RNA sequencing reveals BMP4 as a basis for the dual-target treatment of diabetic retinopathy. Dong L et al., 2021 Feb, J Mol Med (Berl)Read more