Anti-Bordetella pertussis cells [scFv 5]

Absolute Antibody

Anti-Bordetella pertussis cells [scFv 5]

7

The scFv version of this antibody was used for an ELISA on filamentous hemagglutinin (FHA), pertactin (PRN) and B. pertussis cells. The antibody was positive very every antigen tested. The scFv version of this antibody was tested for its ability to inhibit B. pertussis binding to HEp-2 cells, normally only 35% of cells do not bind to HEp-2 cells but this antibody increases that rate to 75%-100%. Furthermore, the ability of the scFv version of this antibody to prevent B. pertussis infection in mice. The viral load present in mice after 48h was a lot lower than in normal circumstances (Hussein et al, 2007; pmid:17724067).

Functional Assay, ELISA

Research Use Only

This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.

-20°C,2°C to 8°C

12352203