Anti-Botulinum neurotoxin [MCS6-27]

Absolute Antibody

Anti-Botulinum neurotoxin [MCS6-27]

7

The antibody binds specifically to BoNT/B, a two-chain neurotoxic protein produced by the bacterium Clostridium botulinum. Botulism, an infection of the bacterium, which is commonly spread through contaminated food, can be a fatal disease, and symptoms include a gradual flaccid paralysis. Paralysis is caused by the internalization by endocytosis of the toxin in specific axons, mediated by the attachment of the heavy chain of the protein to axon surface. Once inside the axon, BoNT/B acts by cleaving SNARE proteins, such as VAMP, which are involved in the docking and fusion of neurosecretory vesicles. This prevents the release of the neurotransmitter acetylcholine, resulting in paralysis of the muscles. The monoclonal antibody MCS6-27 binds to the Hc and H5 fragments of BoNT/B, and the binding epitope is located in the heavy chain, between amino acids E859 and E1291. The monoclonal antibody is able to capture BoNT/B at SDS concentrations between 0 and 0.4 mM, and can be used to screen food samples for botulinum toxin.

Western Blot, ELISA

ELISA; WB

Research Use Only

Monoclonal

MCS6-27

Human

IgG1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Bacteria

Clostridium botulinum

Recombinant BoNT/B fragments expressed in E. coli as GST-fusion proteins.

-20°C,2°C to 8°C

12352203