Anti-Botulinum neurotoxin type A [CR1]

Absolute Antibody

Anti-Botulinum neurotoxin type A [CR1]

7

CR1 binds to both BoNT/A1 and BoNT/A2 with high affinity (CR1 scfv: KD BoNT/A1 ~ 0.13 nM; KD BoNT/A2 ~ 0.4 nM. CR1 IgG: KD BoNT/A1 ~ 2.5 pM; KD BoNT/A2 ~ 1.7 nM.). CR1 Fab fragment binds to the BoNT/A1 binding domain, at the interface between the N-termin

Antibody binding can be studied by FC and the antibody also shows in vivo neutralizing activity against botulinum neurotoxin - the neutralizing activity is increased when administered in combination with the human BoNT/A antibodies RAZ1 and 2G11.

Flow Cytometry, Neutralisation/Blocking

Neutralize; FC

Research Use Only

Monoclonal

CR1

bont

botulinum neurotoxin type A

botulinum neurotoxin type A; CBO_RS04150; CBO0806

Human

IgG1

Botulinum neurotoxin type A

Bacteria

Clostridium

CR1 was produced by a yeast gene diversity library used to increase the affinitiy of scFv AR2 for BoNT/A2, while maintinaing high affinity binding to BoNT/A2. AR2 was generated after multiple rounds of affinity maturation of the humanized C25, which itself was created by CDR-grafting from the parental murine C25. Murine C25 was prepared by constructing a scFv phage display library from mice immunized with recombinant BoNT/A binding domain (Hc).

-20°C,2°C to 8°C

12352203