Anti-BpaB autotransporter [BpaB#4]

Absolute Antibody

Anti-BpaB autotransporter [BpaB#4]

9

This antibody is specific for Burkholderia mallei autotransporter BpaB. Autotransporter proteins form one of the largest class of virulence factors in Gram-negative organisms and perform important functions in pathogenesis including flocculation, formatio

Given the function of autotransporters, such as BpaB in pathogenesis and their overall structure, they are excellent targets for developing medical countermeasures (MCM) against pathogenic organisms. A significant portion of AT (passenger domain) is readily accessible for recognition by the immune system as it is exposed on the bacterial surface. Consequently, BpaB#4 antibody is recommended as a potential research target in treatment and biosafety measures associated with B. mallei. Efficient and specific binding of this antibody to BpaB was confirmed via Western blot; BpaB#4 (BpaB-MAb#4) identified this autotransporter in the material from whole cell lysates (WCL), total membrane proteins (TMP) and sarkosyl-insoluble fraction containing OM proteins (OMP) coming from recombinant E.coli with B. mallei BpaB (Zimmerman et al., 2015). BpaB was shown to be a promising target for the B. mallei therapies due to its involvement in adherence to host cells, biofilm formation and pathogenicity in a mouse model of glanders (Zimmerman et al., 2015). BpaB#4 antibody was further utilised in the identification of B. mallei via immunostaining in various tissues experimentally infected with the bacterium, expanding the antibodys potential use in therapeutics and diagnostics (Zimmerman et al., 2018).

Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA

Research Use Only

Monoclonal

BpaB#4

Human

IgM

This chimeric human antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

Bacteria

-20°C,2°C to 8°C

12352203