Anti-Brg1 [4E5 ]

Absolute Antibody

Anti-Brg1 [4E5 ]

7

mAb 4E5 specifically recognises alpha-Brg1

ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry

IF; IP; WB; IHC

Research Use Only

Monoclonal

4E5

SMARCA4

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4

ATP-dependent helicase SMARCA4; BAF190; BAF190A; brahma protein-like 1; BRG1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; global transcription activator homologous sequence; homeotic gene regulator; hSNF2b; mitotic growth and transcription activator; MRD16; nuclear protein GRB1; protein brahma homolog 1; protein BRG-1; RTPS2; SNF2; SNF2-beta; SNF2L4; SNF2LB; SNF2-like 4; sucrose nonfermenting-like 4; SWI2; transcription activator BRG1

Rabbit

IgG

Transcription activator BRG1

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Human, Mouse

Human, Mouse

Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freund’s complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5:1 in a 50% polyethylene glycol solution. The resulting hybridoma cells were plated onto 96-wellplates and cultured in HAT selection medium. After 10 days post-fusion, the hybridoma supernatants were screened by means of an ELISA against GST-fused Brg1. Positive clones were subcloned and rescreened by ELISA and immunoblotting.

-20°C,2°C to 8°C

12352203