Anti-Butyrylcholinesterase [C191]

Absolute Antibody

Anti-Butyrylcholinesterase [C191]

7

The antibody selectively captures completely denatured human butyrylcholinesterase. The antibody does not bind native butyrylcholinesterase. The antibody does not recognise mouse butyrylcholinesterase.

The authors developed an antibody that can be used for immunopurifying heat-inactivated, denatured human butyrylcholinesterase. The specificity of the antibody (IgG1 format) for denatured butyrylcholinesterase was confirmed by ELISA analysis.The immobilized antibody was able to selectively extracts denatured butyrylcholinesterase from plasma. The selective removal of completely denatured butyrylcholinesterase by the immobilized antibody was visualized on Western blots. Mass spectrometry confirmed the finding from Western blots that the antibody immunoputifies butyrylcholinesterase from heat-inactivated plasma (Peng et al., 2016; PMID: 28058292).

Western Blot, ELISA

ELISA; WB

Research Use Only

Monoclonal

C191

BCHE

butyrylcholinesterase

acylcholine acylhydrolase; BCHED; butyrylcholine esterase; CHE1; CHE2; choline esterase II; cholinesterase; cholinesterase (serum) 2; cholinesterase 1; E1; pseudocholinesterase

Rabbit

IgG

Cholinesterase

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original antibody was generated by injecting mice with denatured, recombinant human butyrylcholinesterase expressed in Chinese hamster ovary cells. The recombinant butyrylcholinesterase had been denatured and reduced by boiling in 1% sodium dodecyl sulfate containing 5% mercaptoethanol before it was injected into mice.

-20°C,2°C to 8°C

12352203