Immunohistochemical analysis of paraffin-embedded human breast cancer, using c-Kit Antibody (M01335-1) KIT was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KIT Antibody (M01335-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Immunohistochemical analysis of paraffin-embedded human breast cancer, using c-Kit Antibody (M01335-1) KIT was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KIT Antibody (M01335-1)overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-c-Kit Rabbit Monoclonal Antibody
M01335-1
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetKIT
Overview
- SupplierBoster Bio
- Product NameAnti-c-Kit Rabbit Monoclonal Antibody
- Delivery Days Customer9
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDCGF-11
- FormulationLiquid
- Gene ID3815
- Target nameKIT
- Target descriptionKIT proto-oncogene, receptor tyrosine kinase
- Target synonymsCD117; C-Kit; c-Kit protooncogene; mast/stem cell growth factor receptor Kit; MASTC; p145 c-kit; PBT; piebald trait protein; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; soluble KIT variant 1; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
- HostRabbit
- IsotypeIgG
- Protein IDP10721
- Protein NameMast/stem cell growth factor receptor Kit
- Scientific DescriptionBoster Bio Anti-c-Kit Rabbit Monoclonal Antibody catalog # M01335-1. Tested in WB, IHC applications. This antibody reacts with Human.
- ReactivityHuman
- Storage Instruction-20°C
- UNSPSC12352203